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Anti-CD30 [YMSM636]

Ab01044-10.3
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, Other Application
Product group Antibodies
TargetTnfrsf8
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD30 [YMSM636]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used to initiate apoptosis in isolated human peripheral blood eosinophils and thus it might be researched as a potential agent for therapeutic agents for hypereosinophilic diseases useful in the treatment of allergic diseases, respiratory diseases, skin diseases, autoimmune diseases, immunodeficiency diseases, digestive diseases, neoplastic diseases and parasitic infections (US20040156847A1).
  • Applications
    Flow Cytometry, ELISA, Other Application
  • Applications Supplier
    FC; ELISA; activating
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    YMSM636
  • Gene ID21941
  • Target name
    Tnfrsf8
  • Target description
    tumor necrosis factor receptor superfamily, member 8
  • Target synonyms
    Cd30; CD30L receptor; D1S166E; Ki; Ki-1; lymphocyte activation antigen CD30; tumor necrosis factor receptor superfamily member 8
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ60846
  • Protein Name
    Tumor necrosis factor receptor superfamily member 8
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse, Human
  • Reactivity Supplier Note
    This antibody was raised by immunising rats with a mouse CD30 - human IgG1 fusion protein.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tnfrsf8 using anti-Tnfrsf8 antibody (A01225-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf8 antigen affinity purified polyclonal antibody (Catalog # A01225-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf8 at approximately 65KD. The expected band size for Tnfrsf8 is at 65KD.
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