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ProductsBack/CD30 antibody [9L24]

CD30 antibody [9L24]

GTX53434
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetTnfrsf8
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD30 antibody [9L24]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9L24
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID21941
  • Target name
    Tnfrsf8
  • Target description
    tumor necrosis factor receptor superfamily, member 8
  • Target synonyms
    Cd30, D1S166E, Ki, Ki-1, tumor necrosis factor receptor superfamily member 8, CD30L receptor, lymphocyte activation antigen CD30
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDQ60846
  • Protein Name
    Tumor necrosis factor receptor superfamily member 8
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tnfrsf8 using anti-Tnfrsf8 antibody (A01225-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf8 antigen affinity purified polyclonal antibody (Catalog # A01225-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf8 at approximately 65KD. The expected band size for Tnfrsf8 is at 65KD.
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