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ProductsBack/Anti- CD31 [BAG 88C8]

Anti- CD31 [BAG 88C8]

AB01517-10.3-BT
Absolute Antibody
TargetPECAM1
Product group Antibodies
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti- CD31 [BAG 88C8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The clone BAG 88C8 is recommended for the identification and analysis of CD31.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BAG 88C8
  • Gene ID5175
  • Target name
    PECAM1
  • Target description
    platelet and endothelial cell adhesion molecule 1
  • Target synonyms
    CD31; CD31 antigen; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP16284
  • Protein Name
    Platelet endothelial cell adhesion molecule
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31/PECAM1 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31/PECAM1 at approximately 90 kDa, 130 kDa. The expected band size for CD31/PECAM1 is at 82 kDa.
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