Anti-CD32 [IV.3]

Absolute Antibody

Anti-CD32 [IV.3]

7

This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic FCGR2A mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).

ImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

IP; Inhibit; IF; Block; ELISA

Research Use Only

Monoclonal

IV.3

FCGR2A

Fc fragment of IgG receptor IIa

CD32; CD32A; CDw32; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc gamma receptor RIIa3; FCG2; fc-gamma-RIIa; FcGR; FCGR2; FCGR2A1; fcRII-a; IGFR2; igG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a

Human

IgG1

Low affinity immunoglobulin gamma Fc region receptor II-a

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human

The original antibody was generated against the FcR of K562 cells.

-20°C,2°C to 8°C

12352203