Anti-CD35 [HB-8592]

Absolute Antibody

Anti-CD35 [HB-8592]

7

This antibody is specific for Human CR1 but does not compete with clones 7G9 or E11; it binds to a site distinct from the C3b binding site and can be used to measure the number of receptors despite occupancy by ligand. This antibody cross-reacts with Chim

This antibody has been used for the analysis of the expression of the CR1 receptor on macrophages isolated from the peritoneal effluents of CAPD patients with peritonitis and human leukemia-derived CD4+ T cell lines by flow cytometry (Hart et al., 1992; PMID: 1606734) (Yaskanin and Waxman, 1995; PMID: 7758124). This antibody was used in immune adherence (IA) binding and release assays (Edberg et al., 1992; PMID: 1376254). This antibody was used in in vivo clearance experiments (Taylor et al., 1997; PMID: 9000042). An scFv version of this antibody was generated, its pharmacokinetics were evaluated, and it was shown to bind mouse mixed lymphocytes and bone marrow-derived dendritic cells in an ELISA and to bind CR1-expressing mouse bone marrow-derived dendritic cells via immunofluorescence labeling (Knight et al., 2008; PMID: 18385459).

Flow Cytometry, ImmunoFluorescence, ELISA, Other Application

FC;

Research Use Only

Monoclonal

HB-8592

CR1

complement C3b/C4b receptor 1 (Knops blood group)

C3b/C4b receptor; C3-binding protein; C3BR; C4BR; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement receptor 1; complement receptor type 1; KN; Knops blood group antigen

Mouse

IgG1

Complement receptor type 1

Human, Monkey, Mouse, Primate

Human

Animals were immunized with purified CR1 from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells.

-20°C,2°C to 8°C

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