Anti-CD35 [HB-8592]

Absolute Antibody

Anti-CD35 [HB-8592]

7

This antibody has been used for the analysis of the expression of the CR1 receptor on macrophages isolated from the peritoneal effluents of CAPD patients with peritonitis and human leukemia-derived CD4+ T cell lines by flow cytometry (Hart et al., 1992; PMID: 1606734) (Yaskanin and Waxman, 1995; PMID: 7758124). This antibody was used in immune adherence (IA) binding and release assays (Edberg et al., 1992; PMID: 1376254). This antibody was used in in vivo clearance experiments (Taylor et al., 1997; PMID: 9000042). An scFv version of this antibody was generated, its pharmacokinetics were evaluated, and it was shown to bind mouse mixed lymphocytes and bone marrow-derived dendritic cells in an ELISA and to bind CR1-expressing mouse bone marrow-derived dendritic cells via immunofluorescence labeling (Knight et al., 2008; PMID: 18385459).

Flow Cytometry, ImmunoFluorescence, ELISA, Other Application

FC;

Research Use Only

Monoclonal

HB-8592

CR1

complement C3b/C4b receptor 1 (Knops blood group)

C3b/C4b receptor; C3-binding protein; C3BR; C4BR; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement receptor 1; complement receptor type 1; KN; Knops blood group antigen

Mouse

IgG1

Complement receptor type 1

Human

Animals were immunized with purified CR1 from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells.

-20°C,2°C to 8°C

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