Anti-CD4 [6G5 (Zanolimumab)]

Absolute Antibody

Anti-CD4 [6G5 (Zanolimumab)]

9

This antibody binds human CD4.

Zanolimumab (clone 6G5, HuMax-CD4, MDX CD4, MDX-016) was among the first fully human mAbs generated from human Ig transgenic mice. The antigen specificity of this antibody was determined using ELISA (PMID: 9631008). The mAb 6G5 was subsequently re-expressed in CHO cells without altering its binding or functional characteristics and renamed HM6G. The avidity of 6G5 for native CD4 expressed on human and nonhuman primate lymphocytes was examined in a flow cytometry assay (PMID: 10444358). Zanolimumab inhibitsCD4+T cells by combining signaling inhibition with potent induction of Fc-mediated effector functions. T cell activation is inhibited by a fast dual mechanism in which the antibody abrogates signaling via the T cell receptor (TCR) and, in addition, down regulates T cell activation by transmission of direct inhibitory signals. In addition, zanolimumab induces killing of CD4+ T cells via antibody-dependent cellular cytotoxicity (PMID: 17942927). Zanolimumab exhibits cytotoxic and anti-proliferative effects and has shown efficacy in T-cell lymphomas. A clinical efficacy and safety study of treatment with weekly dose of 980 mg of HuMax-CD4 in 8 patients having refractory or relapsed CD4+ peripheral T-cell lymphoma (PTCL) of non-cutaneous type revealed that Zanolimumab is well tolerated (PMID: 20629661). Zanolimumab has also been reported to cause depletion of CD4+ T cells from human skin in a human psoriasis xenograft mouse model (PMID: 17091277).

Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

6G5 (Zanolimumab)

CD4

CD4 molecule

CD4 antigen (p55); CD4 receptor; CD4mut; IMD79; OKT4D; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Mouse

IgG2b

T-cell surface glycoprotein CD4

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Monkey, Primate

-20°C,2°C to 8°C

12352203