Anti-CD4 [OKT4A]

Absolute Antibody

Anti-CD4 [OKT4A]

9

This antibody binds a polymorphic OKT4 epitope on the CD4 molecule. The polymorphism results due to a cytosine-to-thymidine transition occurring at nucleotide position 868, which causes a tryptophan residue to be replaced with an arginine residue at posit

This antibody was used for flow cytometry on human peripheral blood mononuclear cells (Bach et al, 1981; pmid:6790623). This antibody was used in a complement- (C) mediated lysis of T cells (Bach et al, 1981; pmid:6790623). A humanized version of this antibody was used for flow cytometry on Human PBM. This was to test the binding affinity of the newly humanized antibody (Pulito et al, 1996; pmid:8609403). Regions of adult human brain tissue were obtained from a 31-year-old man at autopsy was infected with HIV and then immunostained with this antibody (Jordan et al ,1990; pmid:1702842). The effect of this antibody on immunosuppression in transplant patients was tested. The experiment was preformed in cynomolgus monkeys (Mourad et al, 1998; pmid:9521196). Immunoperoxidase was preformed with this antibody on frozen sections of lymph nodes and sequentially obtained allograft biopsies. The tissues used were obtained from cynomolgus monkeys (Mourad et al, 1998; pmid:9521196). The infection of HIV-1 was determined after binding to this antibody (Zeira et al, 1991; pmid:2034680). MT-4, U-937, and PBL cells were immunostained with this antibody (Schols et al, 1988; pmid:2566170). This antibody was used for treatment of chronic plaque psoriasis (Gottlieb et al, 2000; pmid:11004613).

Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry

Research Use Only

Monoclonal

OKT4A

CD4

CD4 molecule

CD4 antigen (p55); CD4 receptor; CD4mut; IMD79; OKT4D; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Mouse

IgG2a

T-cell surface glycoprotein CD4

Human

-20°C,2°C to 8°C

12352203