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Anti-CD40 [G28.5]

Ab00129-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, Other Application
Product group Antibodies
TargetCD40
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD40 [G28.5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody detects CD40 by immunostaining. The original mouse IgG1 format was shown to stimulate B-cells (Bishop 2012 and references therein).
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, Other Application
  • Applications Supplier
    FC; IP; IHC; activate
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    G28.5
  • Gene ID958
  • Target name
    CD40
  • Target description
    CD40 molecule
  • Target synonyms
    B cell surface antigen CD40; B cell-associated molecule; Bp50; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDW40; p50; TNFRSF5; tumor necrosis factor receptor superfamily member 5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP25942
  • Protein Name
    Tumor necrosis factor receptor superfamily member 5
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Human erythrocyte-rosette-negative tonsillar lymphocytes.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10051) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 43 kDa. The expected band size for CD40/TNFRSF5 is at 30 kDa.
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