Anti-CD45 (leukocyte common antigen) [YBM/42.2.2]

Absolute Antibody

Anti-CD45 (leukocyte common antigen) [YBM/42.2.2]

9

This antibody recognises a cell surface marker present on all leukocytes but absent from erythroid cells.

Radioactive binding assay tests demonstrated the ability of the YBM/42.2.2 antibody to label mature hemopoietic cells and a variety of cell lines which were neoplastic representatives of progenitor cells (Watt et al., 1983). This antibody was also used to sort CBA bone marrow cells via FACS and the results confirmed the specificity profile acuired in RIA experiments (Watt et al., 1983). Similar, FACS analysis was performed on mouse fetal liver cells as well (Boehm et al., 2014;Watt et al., 1983). Finally, it cao identify leukocytes and is also recommended to enrich for early progenitor cells from mouse fetal liver (which is predominantly populated by erythroid line) (Watt et al., 1983).

Flow Cytometry, ImmunoPrecipitation, RadioImmunoAssay

Research Use Only

Monoclonal

YBM/42.2.2

Ptprc

protein tyrosine phosphatase, receptor type, C

CD45; Lca; L-CA; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; protein tyrosine phosphatase, receptor-type, c polypeptide; receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein

Mouse

IgG2b

Receptor-type tyrosine-protein phosphatase C

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203