Anti-CD47 [C5D5]

Absolute Antibody

Anti-CD47 [C5D5]

7

C5/D5 is recommended for staining cell surface CD47 as well as neutralising CD47. Furthermore, it is capable of inhibiting peripheral mononuclear neutrophils migration across polarised monolayers of the human intestinal epithelial cell line T84 in a bidirectional fashion (Parkos et al., 1996). This antibody is also very effective in detecting CD47 in flow cytometry; this was demonstrated with various cells, such as CaCO2 and polymorphonuclear neutrophils (Liu et al., 2001). Finally, C5D5 was also utilised in the immunofluorescence staining of the basolateral surfaces of methanol-fixed CaCO2 monolayers expressing exogenously transfected human CD47 (Liu et al., 2001).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

FC; functional inhibition; IF; IP; ELISA

Research Use Only

Monoclonal

C5D5

CD47

CD47 molecule

antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; integrin-associated signal transducer; leukocyte surface antigen CD47; MER6; OA3; Rh-related antigen

Mouse

IgG2b

Leukocyte surface antigen CD47

Human

This antibody was raised by immunising Balb/c mice with T84 epithelial membranes.

-20°C,2°C to 8°C

12352203