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Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb91440 antibody. Corresponding CD47 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb91440 antibody. Corresponding CD47 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb91440 antibody. Corresponding CD47 RNA-seq data are presented for the same tissues.

Anti-CD47 Antibody

AMAB91440
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD47
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CD47
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL6913
  • Conjugate
    Unconjugated
  • Gene ID961
  • Target name
    CD47
  • Target description
    CD47 molecule
  • Target synonyms
    IAP, MER6, OA3, leukocyte surface antigen CD47, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, Rh-related antigen, antigen identified by monoclonal antibody 1D8, antigenic surface determinant protein OA3, integrin associated protein, integrin-associated signal transducer
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ08722
  • Protein Name
    Leukocyte surface antigen CD47
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CD47 using anti-CD47 antibody (A00360-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD47 antigen affinity purified polyclonal antibody (Catalog # A00360-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD47 at approximately 41KD. The expected band size for CD47 is at 35KD.
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