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Anti-CD48 [OX-78]

Ab00574-2.0
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking
Product group Antibodies
TargetCd48
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD48 [OX-78]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds CD48, and has been used in immunoprecipitation and blocking studies to identify and characterise CD48, the ligand for murine CD2 (Kato, 1992). This antibody is able to block the binding of a chimeric molecule comprised of the extracellular domain of mouse CD2 and human IgG1 Fc to T cell lines at the ligand site.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking
  • Applications Supplier
    FC; IP; block
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-78
  • Gene ID12506
  • Target name
    Cd48
  • Target description
    CD48 antigen
  • Target synonyms
    AI449234; AW610730; BCM1; Bcm-1; BCM1 surface antigen; BLAS; BLAST; BLAST1; BLAST-1; CD48 antigen; HM48-1; MEM-102; MRC OX-45 surface antigen; Sgp-; Sgp-60; signaling lymphocytic activation molecule 2; SLAM family member 2; SLAMF2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP18181
  • Protein Name
    CD48 antigen
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    OX-78 was prepared by immunising rats against mouse CD48.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd48 using anti-Cd48 antibody (A03281-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd48 antigen affinity purified polyclonal antibody (Catalog # A03281-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd48 at approximately 50 kDa. The expected band size for Cd48 is at 28,45-50 kDa.
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