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ProductsBack/anti-CD49d / LPAM1 / Integrin alpha 4 antibody [DATK32] (PE)

anti-CD49d / LPAM1 / Integrin alpha 4 antibody [DATK32] (PE)

ARG21044
Arigo Biolaboratories
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetItga4
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-CD49d / LPAM1 / Integrin alpha 4 antibody [DATK32] (PE)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DATK32
  • Concentration
    0.1 mg/ml
  • Conjugate
    RPE
  • Gene ID16401
  • Target name
    Itga4
  • Target description
    integrin alpha 4
  • Target synonyms
    CD49D, Itga4B, integrin alpha-4, CD49 antigen-like family member D, LPAM subunit alpha, VLA-4 receptor, alpha 4 subunit, VLA-4 subunit alpha, integrin alpha-IV, integrin alpha4B, lymphocyte Peyer patch adhesion molecules subunit alpha
  • Host
    Rat
  • Isotype
    IgG2a
  • Scientific Description
    The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008]
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01493-02 Integrin alpha 4 + beta 7 antibody [DATK32] (Biotin). Right panel : co-stained with Integrin alpha 4 + beta 7 antibody [DATK32] (Biotin) and Mouse CD45R antibody (APC) Left panel : co-stained with isotype control and Mouse CD45R antibody (APC) antibody amount : 0.25 microg (5 microl)
Product group Antibodies
References
GeneTex
Integrin alpha 4 + beta 7 antibody [DATK32] (Biotin)GTX01493-02
ApplicationsFlow Cytometry
ReactivityMouse
TargetItga4
  • SizePrice
Figure 1. Western blot analysis of Integrin Alpha 4/Itga4 using anti-Integrin Alpha 4/Itga4 antibody (A00468-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 4/Itga4 antigen affinity purified polyclonal antibody (Catalog # A00468-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 4/Itga4 at approximately 150-180 kDa. The expected band size for Integrin Alpha 4/Itga4 is at 116 kDa.
Product group Antibodies
Boster Bio
Anti-Integrin Alpha 4/Itga4 Antibody Picoband(r)A00468-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse, Rat
TargetItga4
  • SizePrice