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Anti-CD5 [OX-19]

AB00554-1.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
ReactivityRat
TargetCd5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD5 [OX-19]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody can be used for denaturing and non-denaturing WB and developed using peroxidase-conjugated rabbit anti-mouse. It can also be used for IHC of cryostat sections from adult rat liver. Staining is also observed on the brush border of the small intestine and colonic epithelium, T cell areas of lymphoid cells but not B cell follicles
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-19
  • Gene ID54236
  • Target name
    Cd5
  • Target description
    Cd5 molecule
  • Target synonyms
    T-cell surface glycoprotein CD5, CD5 antigen, Lymphocyte antigen CD5, T-cell surface glycoprotein CD5-like
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP51882
  • Protein Name
    T-cell surface glycoprotein CD5
  • Reactivity
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (M00480-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD5 antigen affinity purified monoclonal antibody (Catalog # M00480-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 67KD
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