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Anti-CD5 [UCHT2]

Ab00693-23.0
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCD5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD5 [UCHT2]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds CD5 and is useful for detection of T cells in peripheral blood and lymph nodes and categorisation of T cell lymphomas and B cell chronic lymphocytic leukaemia.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    FC; IHC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    UCHT2
  • Gene ID921
  • Target name
    CD5
  • Target description
    CD5 molecule
  • Target synonyms
    CD5 antigen (p56-62); epididymis secretory sperm binding protein; LEU1; lymphocyte antigen T1/Leu-1; T1; T-cell surface glycoprotein CD5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06127
  • Protein Name
    T-cell surface glycoprotein CD5
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Thymocytes followed by Sezary T cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (A00480-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (Catalog # A00480-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67 kDa. The expected band size for CD5 is at 54 kDa.
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