Mouse anti Human CD5, conjugated with FITC

Nordic-MUbio

Mouse anti Human CD5, conjugated with FITC

7

PBMC: Add10 microl of MAB/106 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

Monoclonal

M28623

FITC

CD5

CD5 molecule

CD5 antigen (p56-62); epididymis secretory sperm binding protein; LEU1; lymphocyte antigen T1/Leu-1; T1; T-cell surface glycoprotein CD5

Mouse

IgG2a

T-cell surface glycoprotein CD5

T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1

See expiration date on vial

Human

Human

CD5=Derived from the hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human t-acute lymphoblastic leukemia (ALL) cells.

2°C to 8°C

12352203