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Anti-CD5 [YTS 121.5.2]

AB00208-2.3-VPB
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCd5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD5 [YTS 121.5.2]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    YTS 121.5.2
  • Gene ID12507
  • Target name
    Cd5
  • Target description
    CD5 antigen
  • Target synonyms
    Ly-1, Ly-12, Ly-A, Lyt-1, T-cell surface glycoprotein CD5, lymphocyte antigen 1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP13379
  • Protein Name
    T-cell surface glycoprotein CD5
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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FACS analysis of mouse C57Bl/6 splenocytes using GTX01465-06 CD5 antibody [53-7.3] (FITC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.5 microg (5 microl)
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Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (A00480-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (Catalog # A00480-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD5 at approximately 70 kDa. The expected band size for CD5 is at 54 kDa.
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