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ProductsBack/Anti-CD56 [2MN2B7], Human IgG1, Fc Silent(tm), kappa

Anti-CD56 [2MN2B7], Human IgG1, Fc Silent(tm), kappa

AB04259-10.3-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetNCAM1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD56 [2MN2B7], Human IgG1, Fc Silent(tm), kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The original version of this antibody was used for an ELISA on Staphylococcal protein A (WO2001033225A2).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2MN2B7
  • Gene ID4684
  • Target name
    NCAM1
  • Target description
    neural cell adhesion molecule 1
  • Target synonyms
    CD56, MSK39, NCAM, neural cell adhesion molecule 1, antigen recognized by monoclonal antibody 5.1H11, neural cell adhesion molecule, NCAM
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP13591
  • Protein Name
    Neural cell adhesion molecule 1
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCAM1 using anti-NCAM1 antibody (A00184-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 antigen affinity purified polyclonal antibody (Catalog # A00184-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAM1 at approximately 150KD. The expected band size for NCAM1 is at 150KD.
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