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Anti-CD69 [2.8]

AB03946-1.1
Absolute Antibody
ApplicationsFlow Cytometry, Other Application
Product group Antibodies
TargetCD69
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD69 [2.8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody can be used for the analysis of CD69 expressed on the surface of cell surface using flow cytometry (PMID: 16983725). This antibody was used as a control antibody in in vivo mouse experiments as it does not bind mouse CD69 (PMID: 16983725; 27151919). This antibody was used in a study to show control on vaccinia virus (VACV) infection in both preventive and therapeutic treatment for transgenic mice expressing human CD69. Treatment in human-CD69 transgenic mouse model with the anti-huCD69-2.8 MAb could affect the primary adaptive response to VACV infection by increasing the number of activated T cells in the periphery (PMID: 31315995). In vivo CD69 targeting in mice expressing human CD69 induced rapid and massive mobilization of bone marrow leukocytes, which was inhibited by desensitization to S1P with FTY720. The treatment highly increased LSK and CLP cell proliferation and numbers, both in the periphery and in the bone marrow, and also augmented S1P1 and CXCR4 expression (PMID: 29483712).
  • Applications
    Flow Cytometry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2.8
  • Gene ID969
  • Target name
    CD69
  • Target description
    CD69 molecule
  • Target synonyms
    activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CLEC2C; C-type lectin domain family 2, member C; EA1; early activation antigen CD69; early lymphocyte activation antigen; early T-cell activation antigen p60; GP32/28; leukocyte surface antigen Leu-23; MLR-3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ07108
  • Protein Name
    Early activation antigen CD69
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD69 using anti-CD69 antibody (A00529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: mouse SP2/0 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD69 antigen affinity purified polyclonal antibody (Catalog # A00529-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD69 at approximately 22KD. The expected band size for CD69 is at 22KD.
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