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Anti-CD70 [TAN 1-7]

Ab00816-2.0
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, Neutralisation/Blocking
Product group Antibodies
TargetCd70
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD70 [TAN 1-7]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used to detect mouse CD70, the ligand for CD27. The original rat IgG1 format has been used in vivo to inhibit CD70-CD27 mediated induction of interferon gamma following injection of glycolipid activators .
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, Neutralisation/Blocking
  • Applications Supplier
    Fc; ELISA; IF; Block
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TAN 1-7
  • Gene ID21948
  • Target name
    Cd70
  • Target description
    CD70 antigen
  • Target synonyms
    CD27LG, Cd27l, Tnfsf7, Tnlg8a, CD70 antigen, CD27 ligand, CD27-L, Ki-24 antigen, tumor necrosis factor ligand 8a, tumor necrosis factor ligand superfamily member 7
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    Recombinant mouse CD70 (extracellular domain, aa residues 41-195).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd70 using anti-Cd70 antibody (A02853-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse B16 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd70 antigen affinity purified polyclonal antibody (Catalog # A02853-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd70 at approximately 30 kDa. The expected band size for Cd70 is at 30 kDa.
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