Anti-CD73 [1E9]

Absolute Antibody

Anti-CD73 [1E9]

7

This antibody is specific for human CD73, which is a 70 kD glycophosphatidylinositol (GPI)-linked 5-nucleotidase. This antibody does not show cross reactivity with mouse CD73.

The enzymatic activity of CD73 causes hydrolysis of extracellular nucleotides into membrane permeable nucleosides. This antibody inhibits soluble placental 5-NT enzymatic activity and recognizes lymphocyte CD73 in indirect immunofluorescence and immunoprecipitation assays. This antibody was also used in the study of cellular distribution of CD73 (PMID: 2137649). It is reported that 1E9 induces vigorous T cell proliferation in the presence of subatomic doses of phorbol myristate and F(ab)2 goat anti-mouse Ig (PMID: 2137649, 2550543). This antibody was also used to study the expression of CD73 in normal mammary gland and breast carcinoma cells using immunohistochemistry (PMID: 1989648). This antibody was also used to detect CD73 in COS cells transfected with CD73 cDNA using flow cytometry (PMID: 7595232). Flow cytometry analysis also showed that anti-CD73 1E9 mAb recognized CD73 on the human breast cancer cell lines LM3 and MDA-MB-231, but failed to detect the murine CD73 variant expressed on isolated mouse mononuclear hematopoietic cells(PMID: 24043904). Bispecific antibody generated using 1E9 and mAb AD2 is reported to inhibit the enzymatic activity of CD73 as well as promotes internalization of CD73.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, Neutralisation/Blocking

Inhibit; IF; IP; IHC; FC

Research Use Only

Monoclonal

1e9

NT5E

5'-nucleotidase ecto

5'-NT; 5'-nucleotidase; CALJA; CD73; E5NT; ecto-5'-nucleotidase; eN; eNT; NT; NT5; NTE; Purine 5-Prime-Nucleotidase

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizing mice with highly purified human placental 5'-NT as immunogen.

-20°C,2°C to 8°C

12352203