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Anti-CD73 [YDBYT 208.3]

Ab00371-23.0
Absolute Antibody
TargetNt5e
Product group Antibodies
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD73 [YDBYT 208.3]
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    YDBYT 208.3
  • Gene ID23959
  • Target name
    Nt5e
  • Target description
    5' nucleotidase, ecto
  • Target synonyms
    2210401F01Rik; 5'-NT; 5'-nucleotidase; AI447961; CD73; ecto-5'-nucleotidase; eNT; NT; Nt5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    Murine CD73.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD73/Nt5e using anti-CD73/Nt5e antibody (A02120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD73/Nt5e antigen affinity purified polyclonal antibody (Catalog # A02120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD73/Nt5e at approximately 63KD. The expected band size for CD73/Nt5e is at 63KD.
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