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Anti-CD79b [B29/123]

Ab01333-23.0
Absolute Antibody
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCD79B
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD79b [B29/123]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is recommended for the immunohistochemical and Western blot investigation of B-cells and their cancers which express CD79b. B29/123 antibody recognises specifically CD79b protein whose expression pattern during B-cell development is distinct from CD79a. Consequently, this antibody might be utilised in the investigation of processes associated with characteristic CD79b expression. For instance, one group used it in the immunofluorescence staining of WEHI231 cells transduced with LAPTM5-IRES-GFP in a study on B-cell activation (Ouchida et al., 2010).
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B29/123
  • Gene ID974
  • Target name
    CD79B
  • Target description
    CD79b molecule
  • Target synonyms
    AGM6; B29; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; CD79b antigen (immunoglobulin-associated beta); CD79b molecule, immunoglobulin-associated beta; IGB; Ig-beta; immunoglobulin-associated B29 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40259
  • Protein Name
    B-cell antigen receptor complex-associated protein beta chain
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human, Mouse
  • Reactivity Supplier Note
    This antibody was raised against the sequence G-E-V-K-W-S-V-G-E-H-P-G-Q-E, representing the carboxy-terminus, from amino acid 215 on, of the human and murine B29 polypeptides.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD79B using anti-CD79B antibody (PB9169). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CD79B protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79B antigen affinity purified polyclonal antibody (Catalog # PB9169) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD79B at approximately 45 kDa. The expected band size for CD79B is at 45 kDa.
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