Anti-CD79b [HM79-16]

Absolute Antibody

Anti-CD79b [HM79-16]

7

Reacts with an extracellular epitope of CD79b.

A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry

FC; IP; IF; IHC

Research Use Only

Monoclonal

HM79-16

Cd79b

CD79B antigen

B29; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; Ig; Igb; Igbe; Ig-be; Igbeta; Ig-beta; immunoglobulin-associated B29 protein; immunoglobulin-associated beta

Mouse

IgG1

B-cell antigen receptor complex-associated protein beta chain

This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG2 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).

-20°C,2°C to 8°C

12352203