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Anti-CD86 [BU63]

Ab00859-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCD86
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD86 [BU63]
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; FC; IHC; IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BU63
  • Gene ID942
  • Target name
    CD86
  • Target description
    CD86 molecule
  • Target synonyms
    B7-2, B7.2, B70, CD28LG2, LAB72, T-lymphocyte activation antigen CD86, B-lymphocyte activation antigen B7-2, BU63, CD86 antigen (CD28 antigen ligand 2, B7-2 antigen), CD86 v6, CTLA-4 counter-receptor B7.2, FUN-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP42081
  • Protein Name
    T-lymphocyte activation antigen CD86
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The antibody was raised by immunising mice using the ARH-77 human B-lymphoblastoid cell line. Antibody BU63 was validated to recognise CD86.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD86 using anti-CD86 antibody (A00220-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD86 antigen affinity purified polyclonal antibody (Catalog # A00220-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD86 at approximately 60-80 kDa. The expected band size for CD86 is at 38 kDa.
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