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ProductsBack/Anti-CD89 [14A8 (14.1)]

Anti-CD89 [14A8 (14.1)]

Ab02426-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, ELISA
Product group Antibodies
TargetFCAR
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD89 [14A8 (14.1)]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds human CD89 with a dissociation constant of Kd= 6.8 X10-10M.The binding of this antibody to human CD89 was confirmed with ELISA. This antibody was also capable of recognizing human CD89 expressing cells like human PMNL cells by flow cytometry. This antibody is not capable of blocking binding of IgA to CD89. This antibody can used to immunoprecipitate soluble CD89 (US7192582). A bispecific antibody construct incorporating the variable regions of the anti-gp41 antibody F240 and the anti-CD89 (immunoglobulin A [IgA] receptor) antibody 14A8 was reported to promote destruction of HIV type 1 (HIV-1) by neutrophils (PMID: 18272577). A Fab-like bispecific antibody molecule or Bi-Fab antibody generated from F240scFv and 14A8scFv showed significant antiboy dependent cell-mediated viral inhibition (ADCVI) activity for multiple clade B and clade C isolates by arming the neutrophils to inhibit HIV infection (PMID: 27419146).
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ELISA
  • Applications Supplier
    ELISA; FC; IP; Therapeutic
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    14A8 (14.1)
  • Gene ID2204
  • Target name
    FCAR
  • Target description
    Fc alpha receptor
  • Target synonyms
    CD89, CTB-61M7.2, FcalphaR, FcalphaRI, immunoglobulin alpha Fc receptor, Fc fragment of IgA receptor, Fc fragment of IgA, receptor for
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP24071
  • Protein Name
    Immunoglobulin alpha Fc receptor
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing HCO12 trangene containing mice with recombinant soluble CD89 antigen.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD89/FCAR using anti-CD89/FCAR antibody (A07546-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD89/FCAR antigen affinity purified polyclonal antibody (Catalog # A07546-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD89/FCAR at approximately 32 kDa. The expected band size for CD89/FCAR is at 32 kDa.
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