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Anti-CDCrel-1 [SP18]

Ab00299-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSEPTIN5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CDCrel-1 [SP18]
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SP18
  • Gene ID5413
  • Target name
    SEPTIN5
  • Target description
    septin 5
  • Target synonyms
    CDCREL, CDCREL-1, CDCREL1, H5, HCDCREL-1, PNUTL1, SEPT5, Septin-5, septin-5, cell division control related protein 1, peanut-like 1, platelet glycoprotein Ib beta chain
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ99719
  • Protein Name
    Septin-5
  • Reactivity Supplier
    Human, Rat, Rabbit
  • Reactivity Supplier Note
    Immunoprecipitate from human brain.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEPT5/SEPTIN5 using anti-SEPT5/SEPTIN5 antibody (A11138). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT5/SEPTIN5 antigen affinity purified polyclonal antibody (Catalog # A11138) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT5/SEPTIN5 at approximately 42 kDa. The expected band size for SEPT5/SEPTIN5 is at 42 kDa.
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