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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic  and nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.

Anti-CDR2 Antibody

HPA018151
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCDR2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CDR2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1039
  • Target name
    CDR2
  • Target description
    cerebellar degeneration related protein 2
  • Target synonyms
    CDR62; cerebellar degeneration-related protein 2; cerebellar degeneration-related protein 2, 62kDa; major Yo paraneoplastic antigen; paraneoplastic cerebellar degeneration-associated antigen; Yo; Yo paraneoplastic antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01850
  • Protein Name
    Cerebellar degeneration-related protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDR2 using anti-CDR2 antibody (A02973-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: mouse kidney tissue lysate, lane 2: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDR2 antigen affinity purified polyclonal antibody (Catalog # A02973-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDR2 at approximately 52KD. The expected band size for CDR2 is at 52KD.
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