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Anti-CDX2

Y300117
Applied Biological Materials
ApplicationsWestern Blot
Product group Antibodies
100 ul
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Overview

  • Supplier
    Applied Biological Materials
  • Product Name
    Anti-CDX2
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ml
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Caudal Type Homeobox 2 or CDX2 is encoded by the CDX2 gene. CDX2 is an essential regulator of intestine-specific genes involved in cell growth and differentiation. It is expressed in the nuclei of intestinal epithelial cells and it is also involved in early embryonic development of the intestinal tract. Mutations on the CDX2 gene are associated with intestinal inflammation and tumorigenesis as well as diseases including bladder adenocarcinoma and cystic teratoma. As a tool used in diagnostic surgical pathology, CDX2 is useful as a biomarker for intestinal cancer, specifically for colorectal differentiation in the gastrointestinal tract.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDX2 using anti-CDX2 antibody (A00877-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human DLD1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDX2 antigen affinity purified polyclonal antibody (Catalog # A00877-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDX2 at approximately 45 kDa. The expected band size for CDX2 is at 33 kDa.
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