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Anti-CEA [EB-022]

AB01003-23.0-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetCEACAM5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CEA [EB-022]
  • Delivery Days Customer
    9
  • Application Supplier Note
    EB-022 was used in ELISAs against CEA (CD66e) and NCA (CD66c) to show specificity of binding (unpublished data).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    EB-022
  • Gene ID1048
  • Target name
    CEACAM5
  • Target description
    CEA cell adhesion molecule 5
  • Target synonyms
    carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; meconium antigen 100
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06731
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 5
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEA/CEACAM5 using anti-CEA/CEACAM5 antibody (A00356-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA/CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA/CEACAM5 at approximately 200 kDa. The expected band size for CEA/CEACAM5 is at 77,180-200 kDa.
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