Anti-CEA [T84.66]

Absolute Antibody

Anti-CEA [T84.66]

7

The binding affinity of the original mouse antibody to carcinoembryonic antigen was determined using a competitive radioimmunoassay (PMID: 6187848). 111In (Indacea) labelled version of this antibody was used to image tumors in patients with colorectal cancer (PMID: 3779658). An engineered antibody fragment (VL-VH-CH3, or minibody) generated using this antibody having bivalent binding to carcinoembryonic antigen showed high level targeting of xenografts (PMID: 8674062). A radiolabeled chimeric version of this antibody was used to target breast cancer cells expressing high levels of CEA and it was found that chimeric 86.66 resulted in good tumor localization as well as significant tumor growth inhibition. This antibody was also used to detect CEA expression in Human breast carcinoma cell line, MCF 7, using flow cytometry and immunohistochemistry (PMID: 21597719). The characterization of the chimeric version of this antibody generated by transient expression in tobacco leaves was done using ELISA (PMID: 10500141). A phase 1 clinical radioimmunotherapy trial studying 22 patients with metastatic CEA-producing malignancies involving 90Yttrium-labeled chimeric T86.44 revealed that the antibody was well tolerated, with reversible thrombocytopenia and leukopenia being dose limiting (PMID: 11051230). This antibody was used in the immunohistochemical staining of paraffin sections of primary tumours from 252 consecutive patients with breast carcinomas (PMID: 9635845).

Flow Cytometry, ELISA, ImmunoHistoChemistry, RadioImmunoAssay

ELISA; RIA; FC; IHC; Immunoscintigraphy; immunotherapy

Research Use Only

Monoclonal

T84.66

CEACAM5

CEA cell adhesion molecule 5

carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; meconium antigen 100

Human

IgG1

Carcinoembryonic antigen-related cell adhesion molecule 5

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

The original antibody was generated by immunizing female BALB/c mice subcutaneously with soluble CEA or with the HC84S cell line.

-20°C,2°C to 8°C

12352203