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ProductsBack/Anti-CEA [T84.66]

Anti-CEA [T84.66]

Research Use Only
Ab02833-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry, RadioImmunoAssay
Product group Antibodies
ReactivityHuman
TargetCEACAM5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CEA [T84.66]
  • Delivery Days Customer
    7
  • Antibody Specificity
    This antibody binds the A3 domain of the human carcinoembryonic antigen.
  • Application Supplier Note
    The binding affinity of the original mouse antibody to carcinoembryonic antigen was determined using a competitive radioimmunoassay (PMID: 6187848). 111In (Indacea) labelled version of this antibody was used to image tumors in patients with colorectal cancer (PMID: 3779658). An engineered antibody fragment (VL-VH-CH3, or minibody) generated using this antibody having bivalent binding to carcinoembryonic antigen showed high level targeting of xenografts (PMID: 8674062). A radiolabeled chimeric version of this antibody was used to target breast cancer cells expressing high levels of CEA and it was found that chimeric 86.66 resulted in good tumor localization as well as significant tumor growth inhibition. This antibody was also used to detect CEA expression in Human breast carcinoma cell line, MCF 7, using flow cytometry and immunohistochemistry (PMID: 21597719). The characterization of the chimeric version of this antibody generated by transient expression in tobacco leaves was done using ELISA (PMID: 10500141). A phase 1 clinical radioimmunotherapy trial studying 22 patients with metastatic CEA-producing malignancies involving 90Yttrium-labeled chimeric T86.44 revealed that the antibody was well tolerated, with reversible thrombocytopenia and leukopenia being dose limiting (PMID: 11051230). This antibody was used in the immunohistochemical staining of paraffin sections of primary tumours from 252 consecutive patients with breast carcinomas (PMID: 9635845).
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry, RadioImmunoAssay
  • Applications Supplier
    ELISA; RIA; FC; IHC; Immunoscintigraphy; immunotherapy
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    T84.66
  • Gene ID1048
  • Target name
    CEACAM5
  • Target description
    CEA cell adhesion molecule 5
  • Target synonyms
    carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; meconium antigen 100
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP06731
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 5
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing female BALB/c mice subcutaneously with soluble CEA or with the HC84S cell line.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203