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ProductsBack/Anti-CEACAM5 [NbCEA5], Human IgG1-Fc Fusion,

Anti-CEACAM5 [NbCEA5], Human IgG1-Fc Fusion,

AB04388-10.159-BT
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, Other Application
Product group Antibodies
TargetCEACAM5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CEACAM5 [NbCEA5], Human IgG1-Fc Fusion,
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding of the original version of this antibody (VHH) to CEACAM5 was characterized in an SPR (KD 0.41 nM), ELISA (EC50 of 0.026 nM), and FACS (EC50 of 1.0 nM). In its bi-specific construction with Ab04389 (Anti-EGFR [7D12]), it served as an anchor (US20220242958).
  • Applications
    Flow Cytometry, ELISA, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    NbCEA5
  • Gene ID1048
  • Target name
    CEACAM5
  • Target description
    CEA cell adhesion molecule 5
  • Target synonyms
    carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; meconium antigen 100
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP06731
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 5
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Human CEA lysates probed with Mouse Anti-CEA (B5) Monoclonal Antibody, Unconjugated (bsm-1624M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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Figure 1. Western blot analysis of CEA/CEACAM5 using anti-CEA/CEACAM5 antibody (A00356-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA/CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA/CEACAM5 at approximately 200 kDa. The expected band size for CEA/CEACAM5 is at 77,180-200 kDa.
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