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Anti-CGA Antibody

144-01239
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetCGA
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CGA Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1081
  • Target name
    CGA
  • Target description
    glycoprotein hormones, alpha polypeptide
  • Target synonyms
    CG-ALPHA, FSHA, GPA1, GPHA1, GPHa, HCG, LHA, TSHA, glycoprotein hormones alpha chain, FSH-alpha, LSH-alpha, TSH-alpha, anterior pituitary glycoprotein hormones common subunit alpha, choriogonadotropin alpha chain, chorionic gonadotrophin subunit alpha, chorionic gonadotropin, alpha polypeptide, follicle-stimulating hormone alpha chain, follicle-stimulating hormone alpha subunit, follitropin alpha chain, luteinizing hormone alpha chain, lutropin alpha chain, thyroid-stimulating hormone alpha chain, thyrotropin alpha chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01215
  • Protein Name
    Glycoprotein hormones alpha chain
  • Scientific Description
    CGA Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Chromogranin A/CGA using anti-Chromogranin A/CGA antibody (A00739-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CGA antigen affinity purified polyclonal antibody (Catalog # A00739-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CGA at approximately 17 kDa. The expected band size for Chromogranin A/CGA is at 13 kDa.
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