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Immunohistochemistry analysis in human skeletal muscle and skin tissues using HPA003440 antibody. Corresponding CHCHD10 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human skeletal muscle and skin tissues using HPA003440 antibody. Corresponding CHCHD10 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human skeletal muscle and skin tissues using HPA003440 antibody. Corresponding CHCHD10 RNA-seq data are presented for the same tissues.

Anti-CHCHD10 Antibody

HPA003440
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCHCHD10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CHCHD10
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID400916
  • Target name
    CHCHD10
  • Target description
    coiled-coil-helix-coiled-coil-helix domain containing 10
  • Target synonyms
    C22orf16, FTDALS2, IMMD, MIX17A, N27C7-4, SMAJ, coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial, MIX17 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYQ3
  • Protein Name
    Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHCHD10 using anti-CHCHD10 antibody (A06312). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: monkey heart tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: rat liver tissue lysates, Lane 8: rat skeletal muscle tissue lytsates, Lane 9: mouse heart tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHCHD10 antigen affinity purified polyclonal antibody (Catalog # A06312) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHCHD10 at approximately 15KD. The expected band size for CHCHD10 is at 15KD.
Product group Antibodies
Boster Bio
Anti-CHCHD10 Antibody Picoband(r)A06312-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCHCHD10
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CHCHD10 AntibodyLS-C664551
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetCHCHD10
  • SizePrice
Atlas Antibodies
PrEST Antigen CHCHD10APREST73579
Protein IDQ8WYQ3
  • SizePrice