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Immunohistochemical staining of human hippocampus shows moderate nucleolar positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate nucleolar positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate nucleolar positivity in neuronal cells.

Anti-CHD2 Antibody

HPA060960
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCHD2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CHD2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1106
  • Target name
    CHD2
  • Target description
    chromodomain helicase DNA binding protein 2
  • Target synonyms
    DEE94, EEOC, chromodomain-helicase-DNA-binding protein 2, ATP-dependent helicase CHD2, CHD-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14647
  • Protein Name
    Chromodomain-helicase-DNA-binding protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHD2 using anti-CHD2 antibody (A04079-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD2 antigen affinity purified polyclonal antibody (Catalog # A04079-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD2 at approximately 250 kDa. The expected band size for CHD2 is at 211 kDa.
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