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ProductsBack/Anti-CHD4 [RAB-C211 ]

Anti-CHD4 [RAB-C211 ]

Ab03246-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetCHD4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CHD4 [RAB-C211 ]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The binding specificity of this antibody to human CHD4 was confirmed with ELISA. This antibody was also used in the immunofluorescense staining of 6 human cell lines (PMID: 26290498).
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C211
  • Gene ID1108
  • Target name
    CHD4
  • Target description
    chromodomain helicase DNA binding protein 4
  • Target synonyms
    CHD-4, Mi-2b, Mi2-BETA, SIHIWES, chromodomain-helicase-DNA-binding protein 4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14839
  • Protein Name
    Chromodomain-helicase-DNA-binding protein 4
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibodywas isoltated from an optimised automated phage display library by panning against specific antigens.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHD4 using anti-CHD4 antibody (A01125-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD4 antigen affinity purified polyclonal antibody (Catalog # A01125-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD4 at approximately 260 kDa. The expected band size for CHD4 is at 218 kDa.
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