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Immunohistochemical staining of human astrocytoma shows strong cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human astrocytoma shows strong cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human astrocytoma shows strong cytoplasmic positivity in tumor cells.

Anti-CHI3L1 Antibody

AMAB91778
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCHI3L1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CHI3L1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL12314
  • Conjugate
    Unconjugated
  • Gene ID1116
  • Target name
    CHI3L1
  • Target description
    chitinase 3 like 1
  • Target synonyms
    ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40, hCGP-39, chitinase-3-like protein 1, 39 kDa synovial protein, cartilage glycoprotein 39, chitinase 3-like 1 (cartilage glycoprotein-39)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP36222
  • Protein Name
    Chitinase-3-like protein 1
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CHI3L1 using anti-CHI3L1 antibody (A01283-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHI3L1 antigen affinity purified polyclonal antibody (Catalog # A01283-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHI3L1 at approximately 43KD. The expected band size for CHI3L1 is at 43KD.
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Immunohistochemical staining of human astrocytoma shows strong cytoplasmic positivity in tumor cells.
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Atlas Antibodies
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