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Anti-Chitinase-3-like protein 1 [L3H4], Rabbit IgG, kappa

AB04552-23.0-BT
Absolute Antibody
ApplicationsELISA, Other Application
Product group Antibodies
ReactivityHuman
TargetCHI3L1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Chitinase-3-like protein 1 [L3H4], Rabbit IgG, kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The humanized version of this antibody was used for ELISA on the human YKL-40 protein. The humanized version of this antibody was used for surface plasmon resonance on human YKL-40 (CN116948021A).
  • Applications
    ELISA, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    L3H4
  • Gene ID1116
  • Target name
    CHI3L1
  • Target description
    chitinase 3 like 1
  • Target synonyms
    ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40, hCGP-39, chitinase-3-like protein 1, 39 kDa synovial protein, cartilage glycoprotein 39, chitinase 3-like 1 (cartilage glycoprotein-39)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36222
  • Protein Name
    Chitinase-3-like protein 1
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHI3L1 using anti-CHI3L1 antibody (A01283-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHI3L1 antigen affinity purified polyclonal antibody (Catalog # A01283-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHI3L1 at approximately 43KD. The expected band size for CHI3L1 is at 43KD.
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