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Immunohistochemical staining of human epididymis shows weak cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human epididymis shows weak cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human epididymis shows weak cytoplasmic and membranous positivity in glandular cells.

Anti-CITED1 Antibody

HPA049051
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCITED1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CITED1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4435
  • Target name
    CITED1
  • Target description
    Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 1
  • Target synonyms
    MSG1, cbp/p300-interacting transactivator 1, melanocyte-specific gene 1, melanocyte-specific protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99966
  • Protein Name
    Cbp/p300-interacting transactivator 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CITED1 using anti-CITED1 antibody (A04351-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CITED1 antigen affinity purified polyclonal antibody (Catalog # A04351-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CITED1 at approximately 23 kDa. The expected band size for CITED1 is at 23 kDa.
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