Anti-class I MHC [5H7]

Absolute Antibody

Anti-class I MHC [5H7]

7

The biotinylated antibody was used for a blocking experiment in human PBLs (Smith et al, 1994; pmid:7913109). Flow cytometry was preformed on human PBMC using this antibody (Woodle et al, 1997; pmid:9036961). This antibody was used for immunoprecipitation on C1R and T2. HMy2.C1R (C1R) are HLA-A negative and -B35 low human B-lymphoblastoid cells, and 0.174 X CEMR (T2) are T-B lymphoblastoid hybrid cells that are missing a large region of the MHC, including the genes encoding TAP1 and TAP2 (Dangoria et al, 2002; pmid:11978783).

Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking

blocking; IP; FC

Research Use Only

Monoclonal

5H7

HLA-A

major histocompatibility complex, class I, A

HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLAA; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain

Human

IgG1

HLA class I histocompatibility antigen, A-3 alpha chain

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human

BALB/c mice were immunized by a series of four to six monthly injections of human PBLs, human Jurkat tumor ceIls, or enriched T lymphocytes.

-20°C,2°C to 8°C

12352203