Anti-class I MHC [5H7]

Absolute Antibody

Anti-class I MHC [5H7]

7

This antibody is specific for HLA class I histocompatibility antigen all different chains. Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived pep

The biotinylated antibody was used for a blocking experiment in human PBLs (Smith et al, 1994; pmid:7913109). Flow cytometry was preformed on human PBMC using this antibody (Woodle et al, 1997; pmid:9036961). This antibody was used for immunoprecipitation on C1R and T2. HMy2.C1R (C1R) are HLA-A negative and -B35 low human B-lymphoblastoid cells, and 0.174 X CEMR (T2) are T-B lymphoblastoid hybrid cells that are missing a large region of the MHC, including the genes encoding TAP1 and TAP2 (Dangoria et al, 2002; pmid:11978783).

Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking

blocking; IP; FC

Research Use Only

Monoclonal

5H7

HLA-A

major histocompatibility complex, class I, A

HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLAA; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain

Rabbit

IgG

HLA class I histocompatibility antigen, A-3 alpha chain

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

BALB/c mice were immunized by a series of four to six monthly injections of human PBLs, human Jurkat tumor ceIls, or enriched T lymphocytes.

-20°C,2°C to 8°C

12352203