Anti-Claudin-4 [KM3900]

Absolute Antibody

Anti-Claudin-4 [KM3900]

7

The antibody specifically recognizes the conformational structure and binds to extracellular loop 2 of CLDN4. The antibody does not react with denatured CLDN4 protein. The antibody does not cross react with CLDN3, 5, 6, and 9. Claudin-4 is a tetraspanin t

Cell lysates were prepared from pancreatic cancer cells (Capan-2 and HPAF-II) and CLDN/CHO cells, and subjected to immunoprecipitation analysis. The antibody was shown to specifically precipitate CLDN4. This antibody detected CLDN4 expressed on CHO cells by flow cytometry. Furthermore, binding of the antibody was detected on CLDN4-expressing pancreatic and ovarian cancer cells, but not on negative cells. Moreover, the chimeric IgG1 (KM3934) version of the antibody was made and its antitumor efficacy was evaluated. KM3934 induced dose-dependent antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity in vitro, and significantly inhibited tumor growth in MCAS or CFPAC-1 xenograft SCID mice in vivo (Suzuki et al., 2009; PMID:19555390). The antibody strongly bound to the CLDN4 expressed in breast cancer cells, colorectal cancer cells and prostate cancer cells. The antibody inhibited the cell movement ability of CLDN4 expression cells. The antibody showed neutralizing activity for CLDN4 (US8076458).

Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking

FC; IP; Neutralizing; Therapeutic

Research Use Only

Monoclonal

KM3900

CLDN4

claudin 4

claudin-4; Clostridium perfringens enterotoxin receptor 1; CPER; CPE-R; CPE-receptor; CPETR; CPETR1; hCPE-R; WBSCR8; Williams-Beuren syndrome chromosomal region 8 protein

Human

IgG1

Claudin-4

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated from BXSB mice immunized with pancreatic cancer cells.

-20°C,2°C to 8°C

12352203