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Anti-Claudin-4 [KM3900]

Research Use Only
Ab03415-10.0
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetCLDN4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Claudin-4 [KM3900]
  • Delivery Days Customer
    7
  • Antibody Specificity
    The antibody specifically recognizes the conformational structure and binds to extracellular loop 2 of CLDN4. The antibody does not react with denatured CLDN4 protein. The antibody does not cross react with CLDN3, 5, 6, and 9. Claudin-4 is a tetraspanin t
  • Application Supplier Note
    Cell lysates were prepared from pancreatic cancer cells (Capan-2 and HPAF-II) and CLDN/CHO cells, and subjected to immunoprecipitation analysis. The antibody was shown to specifically precipitate CLDN4. This antibody detected CLDN4 expressed on CHO cells by flow cytometry. Furthermore, binding of the antibody was detected on CLDN4-expressing pancreatic and ovarian cancer cells, but not on negative cells. Moreover, the chimeric IgG1 (KM3934) version of the antibody was made and its antitumor efficacy was evaluated. KM3934 induced dose-dependent antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity in vitro, and significantly inhibited tumor growth in MCAS or CFPAC-1 xenograft SCID mice in vivo (Suzuki et al., 2009; PMID:19555390). The antibody strongly bound to the CLDN4 expressed in breast cancer cells, colorectal cancer cells and prostate cancer cells. The antibody inhibited the cell movement ability of CLDN4 expression cells. The antibody showed neutralizing activity for CLDN4 (US8076458).
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking
  • Applications Supplier
    FC; IP; Neutralizing; Therapeutic
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    KM3900
  • Gene ID1364
  • Target name
    CLDN4
  • Target description
    claudin 4
  • Target synonyms
    claudin-4; Clostridium perfringens enterotoxin receptor 1; CPER; CPE-R; CPE-receptor; CPETR; CPETR1; hCPE-R; WBSCR8; Williams-Beuren syndrome chromosomal region 8 protein
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDO14493
  • Protein Name
    Claudin-4
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated from BXSB mice immunized with pancreatic cancer cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203