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Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.

Anti-CLCN3 Antibody

HPA044723
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCLCN3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CLCN3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1182
  • Target name
    CLCN3
  • Target description
    chloride voltage-gated channel 3
  • Target synonyms
    CLC3, ClC-3, NEDHYBA, NEDSBA, H(+)/Cl(-) exchange transporter 3, chloride channel 3, chloride channel protein 3, chloride channel, voltage-sensitive 3, chloride transporter ClC-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51790
  • Protein Name
    H(+)/Cl(-) exchange transporter 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CLCN3 using anti-CLCN3 antibody (A05300-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SH-SY6Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLCN3 antigen affinity purified polyclonal antibody (Catalog # A05300-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLCN3 at approximately 170 kDa. The expected band size for CLCN3 is at 91 kDa.
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