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Immunohistochemical staining of human cerebellum shows strong immunoreactivity in myelinated neural processes.
Immunohistochemical staining of human cerebellum shows strong immunoreactivity in myelinated neural processes.
Immunohistochemical staining of human cerebellum shows strong immunoreactivity in myelinated neural processes.

Anti-CNP Antibody

AMAB91068
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCNP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CNP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL2871
  • Conjugate
    Unconjugated
  • Gene ID1267
  • Target name
    CNP
  • Target description
    2'',3''-cyclic nucleotide 3'' phosphodiesterase
  • Target synonyms
    CN37, CNP1, HLD20, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2', 3' cyclic nucleotide 3' phosphohydrolase, 2',3'-cyclic nucleotide 3' phosphodiesterase, CNPase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP09543
  • Protein Name
    2',3'-cyclic-nucleotide 3'-phosphodiesterase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysate, Lane 7: mouse brain tissue lysate, Lane 8: mouse Neuro-2a whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNPase/CNP antigen affinity purified polyclonal antibody (Catalog # A01017-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNPase/CNP at approximately 45 kDa. The expected band size for CNPase/CNP is at 45 kDa.
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