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Immunohistochemical staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

Anti-CNTF Antibody

HPA019654
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCNTF
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CNTF
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1270
  • Target name
    CNTF
  • Target description
    ciliary neurotrophic factor
  • Target synonyms
    Ciliary Neuronotrophic Factor; ciliary neurotrophic factor; HCNTF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26441
  • Protein Name
    Ciliary neurotrophic factor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunofluorescence staining of U251 cells with CSB-PA06039A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. IHC analysis of CNTF using anti-CNTF antibody (PB9012). CNTF was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-CNTF Antibody (PB9012) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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