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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Anti-CNTRL Antibody

HPA020480
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCNTRL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CNTRL
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11064
  • Target name
    CNTRL
  • Target description
    centriolin
  • Target synonyms
    CEP1, CEP110, FAN, bA165P4.1, centriolin, 110 kDa centrosomal protein, bA165P4.1 (ortholog of mouse Ma2a8), bA165P4.2 (centrosomal protein 1), centriole associated protein, centrosomal protein 110kDa, centrosomal protein of 110 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z7A1
  • Protein Name
    Centriolin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CEP110/CNTRL using anti-CEP110/CNTRL antibody (A07479-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP110/CNTRL antigen affinity purified polyclonal antibody (A07479-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP110/CNTRL at approximately 269 kDa. The expected band size for CEP110/CNTRL is at 269 kDa.
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