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Immunohistochemical staining of human liver shows strong cytoplasmic and extracellular positivity.
Immunohistochemical staining of human liver shows strong cytoplasmic and extracellular positivity.
Immunohistochemical staining of human liver shows strong cytoplasmic and extracellular positivity.

Anti-COL18A1 Antibody

HPA030933
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCOL18A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-COL18A1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80781
  • Target name
    COL18A1
  • Target description
    collagen type XVIII alpha 1 chain
  • Target synonyms
    GLCC, KNO, KNO1, KS, collagen alpha-1(XVIII) chain, antiangiogenic agent, collagen, type XVIII, alpha 1, endostatin, multi-functional protein MFP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP39060
  • Protein Name
    Collagen alpha-1(XVIII) chain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of COL18A1 using anti-COL18A1 antibody (A01302-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL18A1 antigen affinity purified polyclonal antibody (Catalog # A01302-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL18A1 at approximately 180KD, 20KD. The expected band size for COL18A1 is at 178KD.
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