Anti-Collagen I [AFA], Human IgG1, Fc Silent(tm), kappa

Absolute Antibody

Anti-Collagen I [AFA], Human IgG1, Fc Silent(tm), kappa

9

This antibody is specific for human collagen I, showing high affinity to the GDF sequence (in A2_DGDFY, part of the alpha2(I)C-telopeptide), suggesting it is a critical epitope.

This antibody binds human collagen I and blocks the binding activity of its C-terminal telopeptide region (denoted as CTTR1) consisting of two alpha1(I)C-telopeptides (denoted as alpha1Ct) and one alpha2(I)C-telopeptide (denoted as alpha2Ct). The KD of this antibody and its Fab and scFv with native alpha2Ct present in procollagen I and with synthetic alpha2Ct were 663 pM, 268 nM, 21 pM, 57 nM, and 75nM for full-length AFA/procollagen I, Fab AFA antibody/procollagen I, full-length AFA antibody/synthetic alpha2Ct, Fab AFA antibody/synthetic alpha2Ct, and scFv/procollagen I, respectively (US20200048333A1). A chimeric IgG version of the original IgA version of this antibody was engineered (Fertala et al., 2013; PMID: 23586407). This antibody has been shown to be an effective therapeutic tool for blocking collagen fibril formation in various models (Fertala et al., 2017; PMID: 28972447) (Fertala et al., 2017; PMID: 23586407).

Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

AFA

Human

IgG1

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgA format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203