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ProductsBack/Anti-Collagen I/COL1A1 Antibody Picoband(r)
Figure 1. Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I/COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I/COL1A1 at approximately 130 kDa, 220 kDa. The expected band size for Collagen I/COL1A1 is at 138 kDa.
Figure 1. Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I/COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I/COL1A1 at approximately 130 kDa, 220 kDa. The expected band size for Collagen I/COL1A1 is at 138 kDa.
Figure 1. Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I/COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I/COL1A1 at approximately 130 kDa, 220 kDa. The expected band size for Collagen I/COL1A1 is at 138 kDa.

Anti-Collagen I/COL1A1 Antibody Picoband(r)

PA2140-2
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCol1a1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Collagen I Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    IHP, IHF, ICC, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID12842
  • Target name
    Col1a1
  • Target description
    collagen, type I, alpha 1
  • Target synonyms
    Col1a-1, Cola-1, Cola1, Mov-13, Mov13, collagen alpha-1(I) chain, alpha-1 type 1 collagen, alpha-1 type I collagen, procollagen, type I, alpha 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11087
  • Protein Name
    Collagen alpha-1(I) chain
  • Scientific Description
    Boster Bio Anti-Collagen I/COL1A1 Antibody catalog # PA2140-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity Supplier
    Human, Mouse, Rat, Hamster
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The E, Zhai Y, Yao Q, et al. Molecular Interaction of Soluble Klotho with FGF23 in the Pathobiology of Aortic Valve Lesions Induced by Chronic Kidney Disease. Int J Biol Sci. 2024,20(9):3412-3425. doi: 10.7150/ijbs.92447
    Read this paper
  • Sharma A, Hill KE, Schwarzbauer JE. Extracellular matrix composition affects outgrowth of dendrites and dendritic spines on cortical neurons. Front Cell Neurosci. 2023,17:1177663. doi: 10.3389/fncel.2023.1177663
    Read this paper
  • Lombardi F, Augello FR, Artone S, et al. Efficacy of probiotic Streptococcus thermophilus in counteracting TGF-β1-induced fibrotic response in normal human dermal fibroblasts. J Inflamm (Lond). 2022,19(1):27. doi: 10.1186/s12950-022-00324-9
    Read this paper
  • Zhang P, The E, Luo Z, et al. Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activity. Mol Med. 2022,28(1):5. doi: 10.1186/s10020-022-00433-4
    Read this paper
  • Hill KE, Lovett BM, Schwarzbauer JE. Heparan sulfate is necessary for the early formation of nascent fibronectin and collagen I fibrils at matrix assembly sites. J Biol Chem. 2022,298(1):101479. doi: 10.1016/j.jbc.2021.101479
    Read this paper
  • Rodríguez-Escamilla JC, Medina-Reyes EI, Rodríguez-Ibarra C, et al. Food-grade titanium dioxide (E171) by solid or liquid matrix administration induces inflammation, germ cells sloughing in seminiferous tubules and blood-testis barrier disruption in mice. J Appl Toxicol. 2019,39(11):1586-1605. doi: 10.1002/jat.3842
    Read this paper
  • Iskenderian A, Liu N, Deng Q, et al. Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone. Skelet Muscle. 2018,8(1):34. doi: 10.1186/s13395-018-0180-z
    Read this paper

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Figure 1. Western blot analysis of COL1A1 using anti-COL1A1 antibody (PB9939). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL1A1 antigen affinity purified polyclonal antibody (Catalog # PB9939) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL1A1 at approximately 138-180 kDa. The expected band size for COL1A1 is at 138 kDa.
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