IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Human Intestinal Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Human Intestinal Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Collagen I Antibody
PA2140-2
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-Collagen I Antibody
- Delivery Days Customer9
- Applications SupplierIHP, IHF, ICC, WB, IHC
- CertificationResearch Use Only
- ConcentrationAdd 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Estimated PurityImmunogen affinity purified.
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- UNSPSC12352203