IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Human Intestinal Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Human Intestinal Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Collagen I/COL1A1 Antibody Picoband(r)
PA2140-2
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetCol1a1
Overview
- SupplierBoster Bio
- Product NameAnti-Collagen I Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierIHP, IHF, ICC, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID12842
- Target nameCol1a1
- Target descriptioncollagen, type I, alpha 1
- Target synonymsalpha-1 type 1 collagen; alpha-1 type I collagen; Co; Col; Col1; Col1a-1; Cola1; Cola-1; collagen alpha-1(I) chain; Mov-; Mov13; Mov-13; procollagen, type I, alpha 1
- HostRabbit
- IsotypeIgG
- Protein IDP11087
- Protein NameCollagen alpha-1(I) chain
- Scientific DescriptionBoster Bio Anti-Collagen I/COL1A1 Antibody catalog # PA2140-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203