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ProductsBack/Anti-Collagen I/COL1A1 Antibody Picoband(r)
Figure 1. Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I/COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I/COL1A1 at approximately 130 kDa, 220 kDa. The expected band size for Collagen I/COL1A1 is at 138 kDa.
Figure 1. Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I/COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I/COL1A1 at approximately 130 kDa, 220 kDa. The expected band size for Collagen I/COL1A1 is at 138 kDa.
Figure 1. Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I/COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I/COL1A1 at approximately 130 kDa, 220 kDa. The expected band size for Collagen I/COL1A1 is at 138 kDa.

Anti-Collagen I/COL1A1 Antibody Picoband(r)

PA2140-2
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCol1a1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Collagen I Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    IHP, IHF, ICC, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID12842
  • Target name
    Col1a1
  • Target description
    collagen, type I, alpha 1
  • Target synonyms
    Col1a-1, Cola-1, Cola1, Mov-13, Mov13, collagen alpha-1(I) chain, alpha-1 type 1 collagen, alpha-1 type I collagen, procollagen, type I, alpha 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11087
  • Protein Name
    Collagen alpha-1(I) chain
  • Scientific Description
    Boster Bio Anti-Collagen I/COL1A1 Antibody catalog # PA2140-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity Supplier
    Human, Mouse, Rat, Hamster
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

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Figure 1. Western blot analysis of COL1A1 using anti-COL1A1 antibody (PB9939). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL1A1 antigen affinity purified polyclonal antibody (Catalog # PB9939) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL1A1 at approximately 138-180 kDa. The expected band size for COL1A1 is at 138 kDa.
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